Membrane protein isolation for western blotting

Amanda Nouwens anouwens at RNA.BIO.MQ.EDU.AU
Sun Jan 24 23:28:51 EST 1999


To:             	protein-analysis at net.bio.net
From:           	Neal Melvin <nrmelvin at acs.ucalgary.ca>
Subject:        	Membrane protein isolation for western blotting
Date sent:      	Sun, 24 Jan 1999 20:19:45 -0700

> Is there a way to selectively isolate/precipitate membrane proteins
> prior to western blotting??
> 

Check out the paper by Molloy et a, 1998 in Electrophoresis 19:837-844  
(Extraction  of membrane proteins by differential solubilization for separation  
using two-dimensional gel electrophoresis). 

The method works very well, and you could use it (or modify) for standard 1D  
gels also.  

Cheers, 

Amanda.  


---------------------------------------------------
Amanda Nouwens
Australian Proteome Analysis Facility (APAF)
Macquarie University
Sydney, NSW 2109
Tel: 02 9850 6209
Fax: 02 9850 6200
Email: anouwens at proteome.org.au



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