activity stain for B-Glucosidase

Randy Cameron rcameron at citrus.usda.gov
Mon Jan 25 10:28:01 EST 1999


rcameron asked

>Would anyone reccomend an activity staining method >for B-glucosidase after
native
>PAGE. Thanks.

DOCTORHIM replyed:

>Use methylumbellyferyl-beta-glucopyranoside from  >Sigma.   Your protein
band ,
>if active,  will fluoresce when viewed on a UV >transilluminator.

Could you provide a reference?  I have questions about concentration,
incubation times, etc.?  Thanks for the help.








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