Lipids off from Protein-Homogenate?

Biology biolo at iss.com
Wed Jan 27 13:33:26 EST 1999


Hi
I have been working on cell extracts of Streptomyces venezuelae, for enzyme
isolation. After sonication and centrifugation at -2 oC, all lipids were
coagulated as particles on the surface, easy to remove. 15000 rpm for 15 min.

MURAT ERAVCI wrote:

> I try to make 2D electrophoresis with rat brain tissue. Can somebody tell me
> how i can get the the lipids off from my Protein homogenate, without
> decreasing the amount of the proteins ?
>
> Thank You in advance






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