couple Inclusion bodies on Sepharose?

Andreas Vogel vogela at uni-muenster.de
Thu Jan 28 04:36:47 EST 1999


Hi all,

I would like to affinity purify  rabbit sera against a protein which I
isolate as overexpressed Inclusion bodies from E.coli. Is it possible to
couple the IB´s on activated CH-sepharose?

Any hints are welcome,

Andreas

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Andreas Vogel                
Department of Biochemistry      
University of Münster       Tel: +49-251-8332128
Wilhelm-Klemm-Str. 2        Fax: +49-251-8333206
D-48149 Münster/Germany     E-mail: vogela at uni-muenster.de
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