CD alpha helix percentage

Dr. Eugene Gussakovsky 03-9683409 vh gussak at agri.gov.il
Thu Jul 1 01:30:04 EST 1999


On Wed, 30 Jun 1999, Peter Prevelige wrote:

> This is a multi-part message in MIME format.
> --------------5D372F1FB607262920564997
> Content-Type: text/plain; charset=us-ascii
> Content-Transfer-Encoding: 7bit
> 
> Far UV CD spectra should be calculated in terms of deg cm2 dmol/ AMINO
> ACID. In this manner, tey are independent of the protein concentration,
> as well as the molecular weight of the protein. Therefore, there is no
> concentration dependent change in helicity, unless there is molecular
> association going on. 
> 
> 
> 
> Young Chai wrote:
> > 
> > Could you tell me  that there are any differences of the percentage of
> > alpha helix from CD spectra with different protein concentration?
> > I appreciate kind answers.
> > 
> > Young Chai
> --------------5D372F1FB607262920564997
> Content-Type: text/x-vcard; charset=us-ascii;
>  name="prevelig.vcf"
> Content-Transfer-Encoding: 7bit
> Content-Description: Card for Peter Prevelige
> Content-Disposition: attachment;
>  filename="prevelig.vcf"
> 
> begin:vcard 
> n:Prevelige;Peter
> tel;fax:205 975-5479
> x-mozilla-html:FALSE
> url:http://www.microbio.uab.edu/faculty/Prevelige/prevelige-p.htm
> org:Univ. of Alabama at Birmingham;Microbiology
> version:2.1
> email;internet:prevelig at uab.edu
> title:Associate Professor
> adr;quoted-printable:;;BBRB 416/6=0D=0A845 19th St. South;Birmingham;AL;35294;USA
> x-mozilla-cpt:;3
> fn:Peter Prevelige
> end:vcard
> 
> --------------5D372F1FB607262920564997--
> 
> 
> 
But a CD spectrum intensity does depend on the protein concentration. So
to calculate a secondary structure from CD, the protein concentration is
necessary while the sendary structure itself is concetration-independent
as Prof.Prevelige noted correctly.

Best wishes.
Dr.Eugene Gussakovsky

---



More information about the Proteins mailing list