Cell lysing for protein assay

[Russell, Rachael]

Hi there
I am looking for protocols cell lysing for Luciferase assays - I have a kit
that has everything but the lysing buffer! If anyone has any ideas, please
let me know. Am currently using an EDTA/PBS/Triton X-100 buffer, but would
like to know of more possibile buffers, and methods ie how long to incubate
buffer. (I am using adherant cells in 96-well plates)

Thanks
Rachael Russell
ESR
Kenepuru Science Centre
New Zealand