Cell lysing for protein assay

Peter pxpst2 at vms.cis.pitt.edu
Wed Jun 9 14:29:53 EST 1999


In article <9173148B8F8CD1118AAC00805F0649C66C2C61 at KSCXCHG2>,
Rachael.Russell at esr.cri.nz ("Russell, Rachael") wrote:

> If anyone has any ideas, please
> let me know. Am currently using an EDTA/PBS/Triton X-100 buffer, but would
> like to know of more possibile buffers, and methods ie how long to incubate
> buffer. (I am using adherant cells in 96-well plates)

20 mM Tris pH 8 with the addition of prtease inhibitors is what I use.
PBS is normal saline so yo will have to use mechanical force to break the
cell open.

regards,

-- 
Peter

_____________________________________________________________________
" Some of you might not agree 
'Cause you probably likes a lot of misery 
But think a while and you will see... 
Broken hearts are for assholes"
                                    FZ



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