how to calculate the molar absorption coefficient of a protein?

Kresten kresten at my-deja.com
Thu Jun 10 03:54:03 EST 1999


> >Is there any web site that would calculate the absorption coefficient
of a

NO (not yet), but there are ways of estimating them

> >protein on the basis of the number of tryptophans, tyrosins, Phe,
disulfide
> >bonds...

> >
>
> See the article: Gill and von Hippel, Analytical Biochemistry 182,
319-326 (1989).

There has also been a more recent paper going down the same way. I
cannot (sorry) find the ref. at the moment.

Also see:
http://www.expasy.ch/tools/protparam.html
and the references at that site.

HTH
Kresten

--
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at LysLeuLeu at crc.dk , transforming the pre at -part into my initials.
Kresten Lindorff Larsen, Dept. Yeast Genetics
Carlsberg Laboratory, Denmark


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