Streptomycin-preciptation

Silke Beismann sbeisma at uni-molgen.gwdg.de
Thu Jun 10 05:07:39 EST 1999


Hi ,

during my protein purification I have a lot of trouble to get rid of
nucleic acids. I have to use high amounts of Benzonase, dialyse
extensively, use several chromatography columns and so on. Now I read in
a paper a protocoll for streptomycin sulfate precipitation of nucleic
acids. Has anyone used this method before? Is it mild to the protein and
efficiently in respect of the nucleic acids?

Thanks for your answers,

Silke Beismann




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