glutathione-sepharose storage

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Jun 22 14:30:03 EST 1999


In article <i.mcfarlane-2206991711210001 at mac017058.guys.icnet.uk>, i.mcfarlane at icrf.icnet.uk (Ian Mc) wrote:
:Hi,
:
:Can anyone tell me what is a good solvent to store glutathione sepharose
:4B columns in after you've finished with them?

Just about anything containing enough something germicidal will work.

:Is PBS and 20% EtOH OK? 

Both OK. Add azide to PBS then. 

:do I need to add a reducing agent (ie reduced
:glutathione) to keep it fresh?

No. 

        - Dima
        



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