Cross-linking protein to a column??
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Wed Jun 23 20:00:44 EST 1999
In article <7kpofb$ec5$1 at dailyplanet.wam.umd.edu>, plato at wam.umd.edu (SNL) wrote:
:I'm looking for information on how to attach a protein of interest that
:binds DNA onto a column, and using the column to purify specific DNA
:Any references appreciated. Anyone know of a kit that can attach protein
:to some sort of bead to make a column?
There are many ways of doing this. Check Sigma, Bio-Rad,
Pharmacia catalogs, "pre-activated sorbents" sections. Also, check
your nearest library for books on "affinity chromatography". It really is
simple: You buy appropriate sorbent and add to it protein solution of
appropriate concentration in appropriate buffer. What is "appropriate"
depends on your exact application and other variables.
More information about the Proteins