wangqm at NIC.BMI.AC.CN
Fri Jun 25 03:52:54 EST 1999
We have expressed a gene in E.Coli, but its molecular weight is not
correct. We use mass spectrum and find it has more 500 dalton than its
putative sequence. After sequencing the protein, we find no incorrect
residue in both end (first 16 residue in N-terminal and the last
residue),comparing with the protein sequence. We purify it from
inclusion body in E.coli.
Who can tell me why?
Much appreciate any help.
Department of Experimental Hematology
Institute of Radiation Medicine
27# Taiping Road
wangqm at nic.bmi.ac.cn
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