CD alpha helix percentage

Peter Prevelige prevelig at uab.edu
Wed Jun 30 18:53:03 EST 1999


Far UV CD spectra should be calculated in terms of deg cm2 dmol/ AMINO
ACID. In this manner, tey are independent of the protein concentration,
as well as the molecular weight of the protein. Therefore, there is no
concentration dependent change in helicity, unless there is molecular
association going on. 



Young Chai wrote:
> 
> Could you tell me  that there are any differences of the percentage of
> alpha helix from CD spectra with different protein concentration?
> I appreciate kind answers.
> 
> Young Chai
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