inclusion body prep

[steven jeffrey drews]

andrew Woods (awoods at PICR.man.ac.uk) wrote:
: I am currently attempting to purify inclusion bodies produced in E.coli
: BL21 (DE3) pLys S.  The purification protocol I have been provided with
: is rather vague and unhelpful.  I would greatly appreciate any protocols
: or references you could forward to me, to this end.

: Thanks in advance

: Andrew Woods

Pierce makes a product called B-Per which gives me a really clean 
inclusion body prep within 2-3 hrs.

Regards
Steve Drews