inclusion body prep

steven jeffrey drews sjdrews at unixg.ubc.ca
Tue Mar 2 17:33:41 EST 1999


andrew Woods (awoods at PICR.man.ac.uk) wrote:
: I am currently attempting to purify inclusion bodies produced in E.coli
: BL21 (DE3) pLys S.  The purification protocol I have been provided with
: is rather vague and unhelpful.  I would greatly appreciate any protocols
: or references you could forward to me, to this end.

: Thanks in advance

: Andrew Woods

Pierce makes a product called B-Per which gives me a really clean 
inclusion body prep within 2-3 hrs.

Regards
Steve Drews




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