inclusion body prep

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Mar 2 21:41:40 EST 1999


In article <7bhp05$n3v$1 at nntp.ucs.ubc.ca>, sjdrews at unixg.ubc.ca (steven jeffrey drews) wrote:
>andrew Woods (awoods at PICR.man.ac.uk) wrote:
>: I am currently attempting to purify inclusion bodies produced in E.coli
>: BL21 (DE3) pLys S.  The purification protocol I have been provided with
>: is rather vague and unhelpful.  I would greatly appreciate any protocols
>: or references you could forward to me, to this end.
>
>: Thanks in advance
>
>: Andrew Woods
>
>Pierce makes a product called B-Per which gives me a really clean 
>inclusion body prep within 2-3 hrs.
>

Just some ionic detergent, as far as I can tell. Probably about 1 M urea too.

        - Dima



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