FPLC with Denaturing Conditions

ross_turbyfill at wrsmtp-ccmail.army.mil ross_turbyfill at wrsmtp-ccmail.army.mil
Wed Mar 3 08:37:39 EST 1999


Does anyone have a protcol for performing FPLC separations (size exclusion)
under denaturing conditions, i.e., in the presence of guanidine HCl, urea,
DTT, etc?  Specifically, are the concentrations of the buffers used in the
chromatography the same concentrations used to denature the proteins (i.e.,
8M Urea or 6M GuHCl)?

Any advice anyone has to offer would be greatly appreciated!!

ross_turbyfill at NOSPAMwrsmtp-ccmail.army.mil



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