Histidine Tetra/Penta and Antibody

Rich Dudley rdudley+ at pitt.edu
Thu Mar 4 09:42:57 EST 1999


guest wrote:

> Hi everyone.
>
> Has anybody used an Tetra-His antibody respectively a Penta-His antibody
> to detect a Tetra/Penta-His-tagged protein?
> Quiagen offers this AB as far as I am informed.
>
> Thanks, Hans

I'm not exactly sure what your question is, but I'll give you my best
effort.  I have used both of Qiagen's antibodies, as well as Invitrogen's,
Clontech's and Babco's for detection of 6-his tagged proteins.  Clontech's
hexa-his antibody gave me the best specificity, but gave a weaker signal
(you can always use a lower dilution, though).  Clontech's Penta-his
antibody gave a good signal, but with a little more background.  Clontech's
antibody was comparable to Qiagen's penta-his in terms of signal and
specificity, Invitrogen and Babco rounded out the list.  I think Babco (now
under a new name) has a different 6-his antobidy available.

Hope this helps!

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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