Histidine Tetra/Penta and Antibody
rdudley+ at pitt.edu
Thu Mar 4 09:42:57 EST 1999
> Hi everyone.
> Has anybody used an Tetra-His antibody respectively a Penta-His antibody
> to detect a Tetra/Penta-His-tagged protein?
> Quiagen offers this AB as far as I am informed.
> Thanks, Hans
I'm not exactly sure what your question is, but I'll give you my best
effort. I have used both of Qiagen's antibodies, as well as Invitrogen's,
Clontech's and Babco's for detection of 6-his tagged proteins. Clontech's
hexa-his antibody gave me the best specificity, but gave a weaker signal
(you can always use a lower dilution, though). Clontech's Penta-his
antibody gave a good signal, but with a little more background. Clontech's
antibody was comparable to Qiagen's penta-his in terms of signal and
specificity, Invitrogen and Babco rounded out the list. I think Babco (now
under a new name) has a different 6-his antobidy available.
Hope this helps!
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
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