How to handle PAS?

Wiebke Schmidt wschmidt at alpha.bio.nat.tu-bs.de
Thu Mar 4 08:32:29 EST 1999


I want to compare the activities of several mutants of an enzyme. In the
assay immunoprecitates of the enzyme are used. I have problems in using
equal amounts of immunoprecipitate in each assay because of the
consistence of the protein A-Sepharose (PAS). Does anyone have a
suggestion how to pipette  equal amounts of PAS?
Thanks in advance,
Wiebke




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