Separation of GPI anchored proteins????
mpiraee at hotmail.com
Fri Mar 5 13:24:56 EST 1999
Since the two components have two very different MWs, it should be better to
use a gel filtration column. They are usually called SPHEROGEL PWHR from
Beckman. They will be separated by a few minutes.
lnd at mail.utexas.edu wrote:
> Hi Netters
> Can someone tell me which HPLC method/column in the best one to separate
> highly glycosolated membrane proteins with GPI-anchore? I tried
> RP-HPLC, but 2 major proteins tend to migrate together. I need to
> separate them for further digestion and peptide mapping.
> Also, those proteins are easily separated by SDS-PAGE (63 and 23 kDa) -
> so I am interested which columns would be the best option to separate
> them based on mol.mass?
> Any ideas?
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