Separation of GPI anchored proteins????

[lnd at mail.utexas.edu]

Hi Netters

Can someone tell me which HPLC method/column in the best one to separate
highly glycosolated membrane proteins with GPI-anchore?  I tried
RP-HPLC, but 2 major proteins tend to migrate together. I need to
separate them for further digestion and peptide mapping.


Also, those proteins are easily separated by SDS-PAGE (63 and 23 kDa)  -
so I am interested which columns would be the best option to separate
them based on mol.mass?

Any ideas?

Thanx

L.D.