Separation of GPI anchored proteins????

lnd at lnd at
Fri Mar 5 11:56:29 EST 1999

Hi Netters

Can someone tell me which HPLC method/column in the best one to separate
highly glycosolated membrane proteins with GPI-anchore?  I tried
RP-HPLC, but 2 major proteins tend to migrate together. I need to
separate them for further digestion and peptide mapping.

Also, those proteins are easily separated by SDS-PAGE (63 and 23 kDa)  -
so I am interested which columns would be the best option to separate
them based on mol.mass?

Any ideas?



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