How to handle PAS?
gbouw at sci.kun.nl
Thu Mar 4 18:00:52 EST 1999
Another idea is to vortex or shake well after each sample or after a few
samples.... that's how I do it, works OK for me!
Wiebke Schmidt <wschmidt at alpha.bio.nat.tu-bs.de> wrote in message
news:36DE8B6D.C164058B at alpha.bio.nat.tu-bs.de...
>I want to compare the activities of several mutants of an enzyme. In the
>assay immunoprecitates of the enzyme are used. I have problems in using
>equal amounts of immunoprecipitate in each assay because of the
>consistence of the protein A-Sepharose (PAS). Does anyone have a
>suggestion how to pipette equal amounts of PAS?
>Thanks in advance,
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