How to handle PAS?

Gerrit Bouw gbouw at
Thu Mar 4 18:00:52 EST 1999

Another idea is to vortex or shake well after each sample or after a few
samples.... that's how I do it, works OK for me!


Wiebke Schmidt <wschmidt at> wrote in message
news:36DE8B6D.C164058B at
>I want to compare the activities of several mutants of an enzyme. In the
>assay immunoprecitates of the enzyme are used. I have problems in using
>equal amounts of immunoprecipitate in each assay because of the
>consistence of the protein A-Sepharose (PAS). Does anyone have a
>suggestion how to pipette  equal amounts of PAS?
>Thanks in advance,

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