N-acetyl transferase purification, What to do next?

lnd at mail.utexas.edu lnd at mail.utexas.edu
Sat Mar 6 13:39:19 EST 1999


If your protein is well soluble in water, I mean if that's a cytosolic one,
so why do not you run that 2-prot fraction on gradient SDS-Gel and cut band
for further in gel digestion/peptide mapping or sequenscing?

You can, once, extract all bands separately and test enzymic activity...so
later will know which one should be digested.

L.D.




More information about the Proteins mailing list