cloning-problem

VVS vvs at sun.ipr.serpukhov.su
Tue Mar 9 10:35:01 EST 1999


By my meance you use too little vector for such large fragment. May be you
could use cosmid or lambda phage...
See Sambrook, Maniatis and Freech (it may be mistakely writing) manual.

>I´ve tried to ligate a 6kb-fragment into a Gal4-Transformation-Vektor
>(p221-4; 12kb long), but failed several times. Does anybody have a
>protocol or reference for such a cloning problem ?? I hope, it´s not
>impossible






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