Protein immobilization on Sepharose using periodate activation (Was: Purifying Antibodies using affinity support)

Warren Gallin wgallin at gpu.srv.ualberta.ca
Tue Mar 9 22:55:44 EST 1999


In Article <7c45t5$rt2$1 at news.doit.wisc.edu>,
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) wrote:
>1. Wash Sepharose CL-4B (not cross-linked version DOES NOT
>work!) with 10-20 volumes of pure water on glass filter.

I checked with Dima, and he clarified something for me.  He means to say
that you must use the CL-4B, which is cross-linked, because plain 4B, which
is not cross linked, will not work with this activation method.

Thanks Dima.

Warren Gallin
Department of Biological Sciences
University of Alberta
Edmonton,  Alberta     T6G 2E9
Canada
wgallin at gpu.srv.ualberta.ca



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