To solubilize transferred proteins..HOW??

lnd at mail.utexas.edu lnd at mail.utexas.edu
Wed Mar 10 12:11:32 EST 1999


Hi  Netters

I have a question here......

Can anyone tell me...if it's possible .... PRACTICALLY....... to get
proteins into solution after being transferred on membrane (NC,
PVDF....whatever..)?  Say....to increase protein purity and
concentration.

I never did that...but ...who knows..maybe you or your friends did?
Say...to chop membrane with certain  protein band,  add
SDS/tween/urea/TX....detergents in buffer....boil....incubate etc.etc.?

Any suggestions would be highly appreciated.

Regards

Levan  Darjania
Austin, TX





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