Western blotting

[Hiranya S. Roychowdhury]

>
>UAB Person wrote:
>
>> I am new to the Western blotting game, and am having some trouble with
>> consistancy from blot to blot.  Can anyone recommend a good Western
>> blotting lab manual with methods, trouble-shooting, recipes etc.  We are
>> attempting to measure GAP-43 in cell culture lysates with a commercial
>> antibody.
>>
>> Thanks in advance.
>>
>> John Moore
>

Very good manuals are avilable from: S&S and Hoefer. Ask them to send these.


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu