Western blotting

[Biology]

hi
see Bi-Rad manual

UAB Person wrote:

> I am new to the Western blotting game, and am having some trouble with
> consistancy from blot to blot.  Can anyone recommend a good Western
> blotting lab manual with methods, trouble-shooting, recipes etc.  We are
> attempting to measure GAP-43 in cell culture lysates with a commercial
> antibody.
>
> Thanks in advance.
>
> John Moore