Western blotting

Rich Dudley rdudley+ at pitt.edu
Mon Mar 15 09:05:17 EST 1999


UAB Person wrote:

> I am new to the Western blotting game, and am having some trouble with
> consistancy from blot to blot.  Can anyone recommend a good Western
> blotting lab manual with methods, trouble-shooting, recipes etc.  We are
> attempting to measure GAP-43 in cell culture lysates with a commercial
> antibody.
>
> Thanks in advance.
>
> John Moore

John--

Go to the top floor of Lister Hill library, and introduce yourself to the
Methods in Enzymology series.  If memory serves, as you're walking south
(towards 8th Ave So.), on the right hand side, ablout halfway down the
stacks.  Far end of the stack, near the study cubes.  Lots of little green
books.

Also, try the Maniatis manual for starters.

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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