bacteria protein expression

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Mar 25 08:39:34 EST 1999


Michael Wing-Yan CHAN <bomchan at ust.hk> wrote:
>      I am expressing a histidine-tagged protein in E.coli
> and I've found that, the protein is secreted out of the the bacteria.
> Do u have any suggestion of how to purify it.

Does the protein in the medium bind to Ni agarose? If yes, you're all
set.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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