Protein extraction from frozen tissues?

Samuel Gray samuel.gray at nottinghnospam.ac.uk
Fri Mar 26 04:22:41 EST 1999


Does anyone have a good protocol for extracting total protein (for western
blotting) from tissues frozen in liquid nitrogen? Should I pulverise the
tissue whilst still frozen (as I would for RNA extraction) or allow the
tissues to defrost and mince/sonicate in a protein extraction buffer?

Thanks for any suggestions,

Samuel Gray.

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_________________________________________________________________________
Samuel Gray
Division of Vascular Medicine,
Derbyshire Royal Infirmary,
Derby,
DE1 2QY
Tel: 01332 254966
Fax: 01332 254968
samuel.gray at nottingham.ac.uk








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