kresten at my-dejanews.com
Tue Mar 30 02:11:48 EST 1999
> Can anyone
> tell me what the effects of iodoacetamide on proteins are?
Iodoacetamide reacts with nucleophiles. Depending on pH this could be
different functional groups. I think Cysteine, Lysine (and N-terminal NH2),
Histidine, Arginine (?) and Tyrosine would all react in their deprotonated
Usually Cysteines (as Cys-S-minus) react first and most.
It could be possible that other nucleophiles would react with the adduct
(forming the dimer/multimer that you refer to) but I find it rather unlikely.
Hope this helps
> Thank you very much for your help.
> Please reply to Claus.Schwechheimer at yale.edu
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