fluorescent adenine analogues

Dr. Peter Gegenheimer PGegen at UKans.nolospamare.edu
Sat May 15 11:32:34 EST 1999


On Wed, 12 May 1999 09:06:45, "Frank Frst" <frank at fuerst.de> wrote:

> Hi,
>
> I am looking for fluorescent derivatives of adenine. I want to try if
> I can measure adenine binding to my protein with one of these.
>
> Unfortunately, I don't even know the names of common fluorescent
> derivatives of adenine - but I know that there are some.
>
> Can anybody help me?
>
> Thanks in advance, Frank

The oldest fluorescent analogue of adenine (& the only one I can think of) is
N(6)-ethenoadenine. The other fluoresecent relatives I can think of are derivatives
of AMP, such as TNP-AMP in which the trinitrophenyl group is attached to the 2'(3')
hydroxyl.

The ethenoadenine-containing nucleotides can be incorporated into poly(A). THere are
also etheno-derivatives of NAD, etc.

o----------------------------------------------------------------------o
| Dr. Peter Gegenheimer       | Vox: 785-864-3939  FAX: 785-864-5321   |
| Department of               |   PGegen at UKans.nospam.edu              |
|   Molecular Biosciences     |   http://rnaworld.bio.ukans.edu/       |
|   & Dept. Evol Biology      |                                        |
| University of Kansas        |"When you have excluded the impossible, |
| 2045 Haworth Hall           |  whatever remains, however improbable, |
| Lawrence  KS  66045-2106    |  must be the truth."      S. Holmes    |
o_____________________________|________________________________________o




More information about the Proteins mailing list