Predicted vs actual MW

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Nov 4 12:55:29 EST 1999


Lee Hunt <L.hunt at sheffield.ac.uk> wrote:
> Does anybody have explanations as to why a protein on a Western can
> differ widely from its predicted size. Other than proteolysis or removal
> of targetting regions what else could cause this?

Some proteins run irregularly on SDS-PAGE, either because they bind
more or less SDS than the "average" protein, or because they do not
completely unfold, even in SDS.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */




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