Predicted vs actual MW

A.F. Simpson AFS7 at
Fri Nov 5 13:08:15 EST 1999

Cornelius Krasel wrote:
> Lee Hunt <L.hunt at> wrote:
> > Does anybody have explanations as to why a protein on a Western can
> > differ widely from its predicted size. Other than proteolysis or removal
> > of targetting regions what else could cause this?
> Some proteins run irregularly on SDS-PAGE, either because they bind
> more or less SDS than the "average" protein, or because they do not
> completely unfold, even in SDS.

Also, heavy glycosilation can cause a protein to run with an abnormally
high molecular weight.

> --Cornelius.


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