Predicted vs actual MW
AFS7 at le.ac.uk
Fri Nov 5 13:08:15 EST 1999
Cornelius Krasel wrote:
> Lee Hunt <L.hunt at sheffield.ac.uk> wrote:
> > Does anybody have explanations as to why a protein on a Western can
> > differ widely from its predicted size. Other than proteolysis or removal
> > of targetting regions what else could cause this?
> Some proteins run irregularly on SDS-PAGE, either because they bind
> more or less SDS than the "average" protein, or because they do not
> completely unfold, even in SDS.
Also, heavy glycosilation can cause a protein to run with an abnormally
high molecular weight.
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