Predicted vs actual MW

Peter pxpst2+ at
Sat Nov 6 09:43:13 EST 1999

In article <3821AEEE.9EFC3D58 at>, Lee Hunt
<L.hunt at> wrote:

> Does anybody have explanations as to why a protein on a Western can
> differ widely from its predicted size. Other than proteolysis or removal
> of targetting regions what else could cause this?

Always remember that PAGE does not seperate purely by mass/size. 
Seperation is by the charge to mass (m/z).  Alter this and and you will
have abherent migration.

Peter Pediaditakis

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