H2O2 assay

Austin P. So (Hae-Jin) haejin at netinfo.ubc.caX
Tue Nov 16 21:10:33 EST 1999


Details...are you using cell extracts? a cell free system? Media?

There are a bunch of substrates that you can use but a few are unstable or
are teratogenic. Fluorescent substrates are by far the best to use in terms
of sensitivity. H2O2 is suprisingly a stable molecule.

If you are looking for in vivo assays, James McNamara over there at Duke
(Epilepsy centre, I think) has done a few of these.

William Tschantz wrote:

> I am looking for a sensitive assay for H2O2 (hydrogen peroxide).  I think
> the enzyme that i am working on may generate it.  I am thinking of
> possible using a peroxidase linked assay.  Has anyone done this before?
> Any help would be appreciated!

--
---
Austin P. So (Hae Jin)

I.I.S.G.P.
Biotechnology Laboratory
University of British Columbia

E-mail: haejin at netinfo.ubc.ca

http://www.interchange.ubc.ca/haejin/index.html (under construction)







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