Coomassie staining of proteins on PVDF

Chris H Lindley chris at scotgate2.demon.co.uk
Wed Nov 17 17:42:57 EST 1999


On Wed, 17 Nov 1999 22:32:11 +0100, thorsten at erfurt.thur.de wrote:
>After SDS-PAGE and Western transfer to PVDF I stained the proteins with
>0.1% Coomassie Blue in H20 dd. To my surprise the band of interest was
>_not_ stained but remained white on a light blue background. Other
>proteins were stained well as expected. Any explanation?  

No sorry:-)  But I've stained pvdf membranes with "Gelcode", and got
Ok results.. (similar to those obtained with amido black staining).
Gelcode is a commercial, one step, gel staining product, and AFAIK
uses coomassie dye. (I could be wrong, as this was in a different
lab and I can;t remeber much about the product).

Cheers
Chris


-- 
ATGCTGCTAGTCGTAGCATGCTGCTTGATCGATGCGGTACGTGATGATCGTAGCTAGCTGGGCTAGTGG
¦  Chris H. Lindley                                  Yorkshire, UK  ¦
¦  chris at scotgate2.demon.co.uk     Ferg on #os/2 and #os2uk, EFnet  ¦
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TACGACGATCAGCATCGTACGACGAACTAGCTACGCCATGCACTACTAGCATCGATCGACCCGATCACC





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