to differentiate between 2 proteins

Achim Recktenwald achimr at home.com
Fri Nov 19 22:57:51 EST 1999


Peptide mapping with subsequent analysis of the fragments by
mass-spectroscopy.

Achim

"Koen Peeters" <kopee at gengenp.rug.ac.be> wrote in message
news:382876A2.24026AF6 at gengenp.rug.ac.be...
>
> Dear,
>
> I need a solution to the following problem : I have a Fab fragment with
> and without C-terminal
> DIKDEL aminoacids produced in plants. I need to know wether the
> C-terminal DIKDEL
> are present or cleaved in planta.
>
> Is there any molecular technique that I could apply that will
> differentiate the presence
> of these 6 C-terminal aminoacids?
>
> No antibodies against DIKDEL/KDEL are working on Western blot.
>
> Thanks
>
> Koen
> --
> ==================================================================
> Koen Peeters
> DEPARTMENT OF GENETICS                         Fax:32 (0)9 2645349
> UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
> Vlaams Instituut voor Biotechnologie                           VIB
> mailto:kopee at gengenp.rug.ac.be       http://www.plantgenetics.rug.ac.be
> ==================================================================
>






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