to differentiate between 2 proteins
achimr at home.com
Fri Nov 19 22:57:51 EST 1999
Peptide mapping with subsequent analysis of the fragments by
"Koen Peeters" <kopee at gengenp.rug.ac.be> wrote in message
news:382876A2.24026AF6 at gengenp.rug.ac.be...
> I need a solution to the following problem : I have a Fab fragment with
> and without C-terminal
> DIKDEL aminoacids produced in plants. I need to know wether the
> C-terminal DIKDEL
> are present or cleaved in planta.
> Is there any molecular technique that I could apply that will
> differentiate the presence
> of these 6 C-terminal aminoacids?
> No antibodies against DIKDEL/KDEL are working on Western blot.
> Koen Peeters
> DEPARTMENT OF GENETICS Fax:32 (0)9 2645349
> UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
> Vlaams Instituut voor Biotechnologie VIB
> mailto:kopee at gengenp.rug.ac.be http://www.plantgenetics.rug.ac.be
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