Coomassie staining of proteins on PVDF

kevin.bailey at nottingham.ac.uk kevin.bailey at nottingham.ac.uk
Wed Nov 24 12:09:04 EST 1999


In article <38331EDB.7CDACC51 at erfurt.thur.de>, Thorsten Burmester
<thorsten at erfurt.thur.de> wrote:

> After SDS-PAGE and Western transfer to PVDF I stained the proteins with
> 0.1% Coomassie Blue in H20 dd. To my surprise the band of interest was
> _not_ stained but remained white on a light blue background. Other
> proteins were stained well as expected. Any explanation?  
> 
		Have you tried it with other stains eg amido black, ponceau red?




More information about the Proteins mailing list