Need help:insoluble to soluble

Frank Fürst ffrank at
Tue Oct 5 03:25:41 EST 1999

Jared Quentin LeMaster schrieb:
> Hi:
>    I expressed foreign protein in E.coli to the level of about 40% total
> protein after IPTG induction. But it is insoluble. Does anyone have any
> experiences that could make it soluble without loss of function or
> expressed as a soluble protein in E.coli?  Pls send your suggestion to
> wang.508 at Thanks a lot.

You can purify it from the insoluble inclusion bodies


Frank Fuerst, Institute of Biochemistry of Potsdam University
Im Biotechnologiepark, D-14943 Luckenwalde
Tel.: +49-3371-681334;   Fax: +49-3371-681339
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