Need help:insoluble to soluble

[Frank Fürst]

Jared Quentin LeMaster schrieb:
> 
> Hi:
>    I expressed foreign protein in E.coli to the level of about 40% total
> protein after IPTG induction. But it is insoluble. Does anyone have any
> experiences that could make it soluble without loss of function or
> expressed as a soluble protein in E.coli?  Pls send your suggestion to
> wang.508 at osu.edu. Thanks a lot.

You can purify it from the insoluble inclusion bodies

Frank

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Frank Fuerst, Institute of Biochemistry of Potsdam University
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