Need help:insoluble to soluble
ffrank at rz.uni-potsdam.de
Tue Oct 5 03:25:41 EST 1999
Jared Quentin LeMaster schrieb:
> I expressed foreign protein in E.coli to the level of about 40% total
> protein after IPTG induction. But it is insoluble. Does anyone have any
> experiences that could make it soluble without loss of function or
> expressed as a soluble protein in E.coli? Pls send your suggestion to
> wang.508 at osu.edu. Thanks a lot.
You can purify it from the insoluble inclusion bodies
Frank Fuerst, Institute of Biochemistry of Potsdam University
Im Biotechnologiepark, D-14943 Luckenwalde
Tel.: +49-3371-681334; Fax: +49-3371-681339
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