Stripping membranes

Amanda Nouwens anouwens at proteome.org.au
Tue Oct 5 17:22:44 EST 1999


Dear Mark,

>Does anyone out there have info on stripping nitrocellulose membranes to
>reprobe with different antibodies? I recall that there was a procedure with
>the chemilluminescent methods, but I forget the details.
>Thanks.

try this method:

wash blot 4 x with PBS (5 min/wash)

incubate for 30 min at 50 deg. C in:

62.5mM Tris-HCl pH 6.8
2% SDS
100mM DTT.

wash 6 x 5 min in PBS.

block as usual.

Cheers,

Amanda Nouwens.







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