anouwens at proteome.org.au
Tue Oct 5 17:22:44 EST 1999
>Does anyone out there have info on stripping nitrocellulose membranes to
>reprobe with different antibodies? I recall that there was a procedure with
>the chemilluminescent methods, but I forget the details.
try this method:
wash blot 4 x with PBS (5 min/wash)
incubate for 30 min at 50 deg. C in:
62.5mM Tris-HCl pH 6.8
wash 6 x 5 min in PBS.
block as usual.
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