phosphopeptide pI and Mr calculation

[Evangelos Christodoulou]

In article <7tud7i$1luk$1 at newssvr04-int.news.prodigy.com> , "PAUL  MACLEAN"
<PM.MACLEAN at prodigy.net> wrote:

> Does anyone know an internet resource that will calculate pI and Mr of
> phosphorylated peptides?
>
> If not, does anyone know the relative pKa's  of the two hydrates oxygen
> molecules of an attached phosphate?  I can calculate the pI and Mr by hand
> if needs be, but I need this information to do so.
>
> Can anyone point me in the right direction? I've checked Pedro's
> biomolecular tools.  Most programs I have found do not handle
> posttranslationally altered amino acids in their pI calculation.
>
> Thank you,
> Paul MacLean
>
>
For the pI:
http://www.embl-heidelberg.de/cgi/pi-wrapper.pl

For the pKa:
http://www.embl-heidelberg.de/ExternalInfo/wade/pub/soft/pka.html

Hope this helps.
E.

--
Evangelos Christodoulou
University of Athens, Greece
If Che Guevara was alive he'd use a Mac
-the only revolutionary-friendly platform