Aggregated protein

Keri Freeland kf94 at columbia.edu
Tue Oct 19 13:19:15 EST 1999


Hi,

     I have a soluble but aggregated protein that I'm trying to prepare
for NMR studies.  I know it's soluble because the protein solution is
clear, and I know it forms aggregates from running size exclusion
columns.

      I'm getting ready to test different buffer conditions (detergents,
salt, pH), but I can't think of a good way to check my samples for
monomeric protein.   The time is more of an issue than amount of
protein, since it expresses well. 

Does anyone know a quick way to do this?

Thanks,
Keri



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