Aggregated protein

Miriam Hirshberg mh270 at mole.bio.cam.ac.uk
Tue Oct 19 16:27:21 EST 1999



Have you tried light scattering, or analytical centrifugation???


M.

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Miriam (Miri) Hirshberg         e-mail: m.hirshberg at mole.bio.cam.ac.uk
Department of Biochemistry      Tel   : (+44) 01223 766018
University of Cambridge         Tel   : (+44) 01223 333600 (switch board)
80 Tennis Court Road            Fax   : (+44) 01223 766002
Old Addenbrooke's Site
Cambridge CB2 1GA, UK

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On Tue, 19 Oct 1999, Keri Freeland wrote:

> Hi,
> 
>      I have a soluble but aggregated protein that I'm trying to prepare
> for NMR studies.  I know it's soluble because the protein solution is
> clear, and I know it forms aggregates from running size exclusion
> columns.
> 
>       I'm getting ready to test different buffer conditions (detergents,
> salt, pH), but I can't think of a good way to check my samples for
> monomeric protein.   The time is more of an issue than amount of
> protein, since it expresses well. 
> 
> Does anyone know a quick way to do this?
> 
> Thanks,
> Keri
> 

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