Aggregated protein

Leonard Pattenden ddlpatte at mailbox.uq.edu.au
Tue Oct 19 18:03:45 EST 1999


G'day Keri;

A *very* rough method is a wavelength scan from 220-320. A highish 310:280
ratio indicates aggregates (the 310 should be almost nothing). The
look of the profile can also be useful. It's not the most accurate method,
but it is quick and is can be surprisingly predictive.

Have a day!

Len...

On Tue, 19 Oct 1999, Keri Freeland wrote:

> Hi,
> 
>      I have a soluble but aggregated protein that I'm trying to prepare
> for NMR studies.  I know it's soluble because the protein solution is
> clear, and I know it forms aggregates from running size exclusion
> columns.
> 
>       I'm getting ready to test different buffer conditions (detergents,
> salt, pH), but I can't think of a good way to check my samples for
> monomeric protein.   The time is more of an issue than amount of
> protein, since it expresses well. 
> 
> Does anyone know a quick way to do this?
> 
> Thanks,
> Keri




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