radiation and glycerol stocks

Marc Saric marc.saric at mpi-dortmund.mpg.de
Mon Oct 25 09:13:59 EST 1999


Wolf schrieb:
> 
> Hello People...
> 
> I just find out that my E.Coli and yeast glycerol stocks in -80
> freezer have been exposed to high radiation by 59Fe for more than  2
> months.  The radioactivity is still very very high.
> 
> Is it possible to have any effects on my glycerol stock from radiation
> such as mutation or whatever possible damage?

Due to the fact that radiation damage is caused by breaking of covalent
bonds and radical-generation from high energy photons, and electrons
(alpha-particles should be shielded by the Eppendorf-tube or Cryo-Vial
in which the stock is enclosed) it seems possible to me that even in a
-80 deg. fridge your DNA might be damaged. Damage might be not as high
as under RT-conditions (I´m not shure) but I would check the stocks and
expect some damage (and mutations following the errors during repair by
the cells own replication/correction-apparatus).



-- 
Bye,

Marc Saric

Max-Planck-Institut für molekulare Physiologie
Otto-Hahn-Strasse 11
44227 Dortmund

phone: +49 231 133 2168



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