Expression of a 300kDa protein possible?

[Christina Philipp]

Hello Ng,

until now, I tried two methods to express my protein:
1. TNT T7 Quick Coupled Transcription/Translation System (Promega).
The luciferase control worked well, but I did not see my desired
protein.
2. A "normal" expression (T7 promoter, induction with 1mM IPTG) in E.
coli HMS174(DE3).

Has anyone ever had success with the expression of such a large
protein?
Thank you for your help.
Christina