fusion protein expression
k-yap at bs.aist-nara.ac.jp
Wed Apr 5 23:00:20 EST 2000
I am a PhD student in Japan. I am facing some problems in expressing my
fusion proteins (both GST-fusions and His-tag fusions) in soluble
fractions; therefore I decided to use the TNT-coupled recticulocyte
system to synthesis the desired proteins.
I need to use these fusion proteins for the following assay:
(i) protein interaction - pull down assay
(ii) in-vitro kinase assay
My questions are:
(i) how to purify the translated product from the TNT- coupled
(ii) Are there any references for the above assays (pull down assay &
in-vitro kinase assay) using both in-vitro translated fusion proteins?
Your help are kindly appreciated. Thank you.
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