"Sticky" FLAG epitopes?

Neil McKenna nmckenna at ottawa.com
Thu Apr 6 14:53:04 EST 2000


Hello,

I am currently using two FLAG-tagged proteins to test for their interaction
properties. It was recently brought to my attention that FLAG tags can be
sticky -- I am wondering now whether the two FLAG tags may increase the
interaction of my two proteins. Does anyone out there have any experience
with this happening? I'm planning on running a control with a separate
epitope, but I'd still like to hear if anyone has any experience with two
FLAG peptides interacting.

Thanks
Neil






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