Protein Precipitation Method

Janna Peters jpeters at ipoe.uni-kiel.de
Mon Apr 17 10:20:55 EST 2000


I want to determine the protein and lipid content of zooplankton from the
same sample. I will extract the lipid with Dichlormethane:Methanol (2:1)
and now I am looking for an adequate protein precipitation method, which I
can combine with the lipid-extraktion without serious influence on the
lipid content.
Has anyone experience with determination of lipid and protein from the same
sample? Maybe the Method of Wessel and Függe 1984 (Analyt. biochem. 138,
141-143) could be useful with some changes?
I would be very thankful for any kind of advice!!!! Thank you in advance.
Janna Peters


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